Rb. Yang et al., 2 MEMBRANE FORMS OF GUANYLYL CYCLASE FOUND IN THE EYE, Proceedings of the National Academy of Sciences of the United Statesof America, 92(2), 1995, pp. 602-606
The cDNAs for two membrane guanylyl cyclases, designated E (GC-E) and
F (GC-F), were isolated from a rat eye cDNA library, Their deduced top
ographic structures correspond to known members of the guanylyl cyclas
e receptor family, containing an extracellular domain, a single membra
ne-spanning domain, a protein kinase-like domain, and a cyclase cataly
tic domain, GC-E was expressed in the eye and the pineal gland, wherea
s GC-F expression was confined to the eye, Overproduction of GC-E and
GC-F in COS cells resulted in expression of guanylyl cyclase activity,
but ligands known to activate other guanylyl cyclase receptors failed
to stimulate enzyme activity, Thus, both GC-E and GC-F remain orphan
receptors. Amino acid sequence similarity between GC-E and GC-F in the
extracellular region and homology with a cyclase expressed in olfacto
ry neurons and retGC, a rod outer-segment-specific cyclase, suggest th
at there is another subfamily of guanylyl cyclase receptors, possibly
restricted to sensory tissues.