A CELL-LINE (MCA-B1) DERIVED FROM A CANINE ORAL ACANTHOMATOUS EPULIS

A canine oral acanthomatous epulis cell line designated MCA-B1 was est ablished in culture. Immunohistochemically the cells reacted intensely with anti-keratin antibody and mildly with anti-vimentin antibody. An ultrastructural examination of the cells confirmed their epithelial n ature. The modal chromosome number was 72 and the cloning efficiency i n soft agar culture ranged from 3.4 to 16.1 per cent with a population doubling time of nine hours. MCA-B1 has since been passaged more than 70 times and maintained in culture for more than a year. Most of the characteristics of the original canine tumour cells were retained in t he isolated cell line.