LEUKOCYTE MARGINATION DURING HEMORRHAGIC-SHOCK CORRELATES TO PRESHOCKMARGINATION AND IS REDUCED BY FUCOIDIN

Systemic and pulmonary circulation kinetics for Cr-51-erythrocytes and In-111-leukocytes were measured in rats during experimental hemorrhag ic shock and normotension with or without pretreatment with the antiro lling agent fucoidin. Leukocyte margination was expressed as transit f actors (white blood cell transit time/red blood cell transit time) for polymorphonuclear and mononuclear cells. There was an increased pooli ng of leukocytes in the pulmonary and systemic vascular beds during sh ock with a maximum after 60 min when the transit factors had increased 2.90-3.72 times in the pulmonary vascular bed and 2.00-3.52 times in the systemic vascular bed for mononuclear and polymorphonuclear cells, respectively. High preshock pooling levels lead to a more pronounced increase in pooling during shock. Pretreatment with fucoidin significa ntly reduced the pooling increase in the systemic vascular bed. Granul ocyte oxidative activity (nitro blue tetrazolium test) invariably incr eased during shock and was not affected by fucoidin.