GROWTH AND INTERFERON-GAMMA PRODUCTION IN BATCH CULTURE OF CHO CELLS

The relationship between growth and interferon-gamma (IFN-gamma) produ ction in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fi tted by the Monod model. The K-s and mu(max) values were found to be 0 .771% (v/v) serum and 0.031 h(-1) respectively. The average specific I FN-gamma production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific prod uction rate and specific growth rate was found in all phases of the cu lture except the lag phase and it was clearly demonstrated that IFN-ga mma production was growth associated. Specific glucose and glutamine u tilisation rates were inversely related to specific growth rates.