LOW-TEMPERATURE CULTIVATION - A STEP TOWARDS PROCESS OPTIMIZATION

Authors
WEIDEMANN R LUDWIG A KRETZMER G
Citation
R. Weidemann et al., LOW-TEMPERATURE CULTIVATION - A STEP TOWARDS PROCESS OPTIMIZATION, Cytotechnology, 15(1-3), 1994, pp. 111-116
Citations number
5
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Cytotechnology → ACNP
ISSN journal
09209069
Volume
15
Issue
1-3
Year of publication
1994
Pages
111 - 116
Database
ISI
SICI code
0920-9069(1994)15:1-3<111:LC-AST>2.0.ZU;2-E
Abstract
Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37 degrees C. Batch and repeated-batch-cultivations in a 2-lit re bioreactor showed a significant influence on metabolism and cell gr owth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate producti on. On the other hand, the maximum cell density and productivity seeme d not to be affected by the temperature reduction.