GLYCOSYLATION AND FUNCTIONAL-ACTIVITY OF ANTI-D SECRETED BY 2 HUMAN LYMPHOBLASTOID CELL-LINES

Cell lines BTSN4 and BTSN5 were produced by the Epstein-Barr Virus (EB V) transformation of B-lymphocytes from the same human donor. Both sec rete an anti-D monoclonal of the IgG1 subclass but these antibodies di splay vastly different effector activities. Specifically, anti-D from BTSN4 has a far greater activity in both monocyte and lymphocyte-media ted ADCC reactions and causes a higher percentage of rosettes to be fo rmed with monocyte-like U937 cells. This variation in functional activ ity is shown to coincide with changes in the structure of the sugar ch ains attached to the asparagine-297 site on the immunoglobulin heavy c hain.