ISOLATION OF A NOVEL COLLAGEN-BINDING PROTEIN FROM THE MUSHROOM, HYPSIZIGUS-MARMOREUS, WHICH INHIBITS THE LEWIS LUNG-CARCINOMA CELL-ADHESION TO TYPE-IV COLLAGEN

A type IV collagen-binding protein of 23 kDa was isolated from the mus hroom, Hypsizigus marmoreus. This protein, HM 23, bound to type TV and type I collagens and gelatin, and to much lesser extent to fibronecti n, but not to laminin or bovine serum albumin. The adhesion of Lewis l ung carcinoma cells was inhibited when the type TV collagen substratum was pretreated with HM 23. A computer search of the determined partia l amino acid sequence indicated no homologous proteins reported. These results indicate that HM 23 is a hitherto undescribed fungus protein that can interact with animal extracellular matrix proteins.