ANALYSIS OF MONOSACCHARIDE COMPOSITION OF MUCIN OLIGOSACCHARIDE-ALDITOLS BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY

Authors
KARLSSON NG HANSSON GC
Citation
Ng. Karlsson et Gc. Hansson, ANALYSIS OF MONOSACCHARIDE COMPOSITION OF MUCIN OLIGOSACCHARIDE-ALDITOLS BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY, Analytical biochemistry, 224(2), 1995, pp. 538-541
Citations number
8
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
224
Issue
2
Year of publication
1995
Pages
538 - 541
Database
ISI
SICI code
0003-2697(1995)224:2<538:AOMCOM>2.0.ZU;2-0
Abstract
A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzat e was re-N-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric d etector. The method was applied on a mixture of neutral oligosaccharid es released from mucin glycopeptides of rat small intestine by alkalin e borohydride. Sugars were detected down to the nanomole range and the results were compared with monosaccharide compositional analysis perf ormed by gas chromatography of acetylated alditols. (C) 1995 Academic Press, Inc.