INTERFERENCE OF SODIUM-AZIDE WITH THE QUANTITATION OF BILIRUBIN - MODIFICATION OF FOG METHOD TO ELIMINATE AZIDE INTERFERENCE

Interference of sodium azide with bilirubin estimation was studied at different concentrations of sodium azide. At an azide concentration (0 .08% or 12.3 mM), 99% inhibition of the color reaction was observed. S imilar results were obtained when the effect of sodium azide was studi ed by fixing bilirubin concentration and increasing the azide concentr ation. The decrease in color reaction can be explained on the basis of competition of the two compounds, i.e., bilirubin and sodium azide fo r diazosulfanilic acid. The method was modified to measure the concent ration of bilirubin in various serum samples containing different amou nts of sodium azide. (C) 1995 Academic Press, Inc.