HUMAN ERYTHROLEUKEMIC (HEL) CELLS EXPRESS A PLATELET P2T-LIKE ADP RECEPTOR

Treatment of Fura-2 loaded HEL cells, a human megakaryocyte-like cell line, with P2-purinoceptor nucleotide ligands (ADP, ATP, UTP, 2-methyl thio-ATP) evoked a rise in cytosolic calcium. Homologous- and cross-de sensitization studies using sequential addition of nucleotides showed that the ADP-induced calcium response in HEL cells was mediated mainly by purinoceptor(s) for which ADP but not ATP was an agonist. There we re also minor contributions for purinoceptors for which ATP and ADP ar e both agonists (probably P2U and P2Y). ATP inhibited the ADP-induced calcium response in HEL cells. This inhibition was overcome by raising the ADP concentration, thus indicating that ATP was an antagonist for the HEL cell ADP receptor. AMP was also an antagonist, albeit weak, f or the HEL cell ADP receptor. Antagonism of the ADP-induced calcium re sponse by ATP was similarly observed in MEG-01 cells, another human me gakaryocyte-like cell line, but not in 293 cells, a nonhematopoietic c ell line. These studies suggest that HEL cells express and ADP recepto r for which ATP and AMP are antagonists. This characteristic of the HE L cell ADP receptor is also displayed by the platelet P2T. Thus, HEL c ells appear to be a surrogate source of the platelet ADP receptor.