Ca. Squier et al., CONTINUOUS-FLOW MUCOSAL CELLS FOR MEASURING THE IN-VITRO PERMEABILITYOF SMALL TISSUE SAMPLES, Journal of pharmaceutical sciences, 86(1), 1997, pp. 82-84
Continuous-flow chambers are described for the measurement of permeabi
lity of small tissue samples. The design incorporates a large-capacity
donor chamber to permit adequate loading of the applied compound and
a low-volume (0.3 mt) receiving chamber that ensures rapid removal of
penetrant at relatively low (1.5 mL/h or less) pumping rates. Differen
t sized support disks allow tissue biopsies as small as 4 mm in diamet
er to be utilized. Comparisons of flux and permeability constants (K-p
) for water across oral mucosa indicate that there was no significant
difference between values obtained for 10- and 4-mm biopsies. Comparis
ons of flux and kb Values for porcine oral mucosa and a synthetic memb
rane between continuous flow and conventional, side-by-side chambers i
ndicated that the latter values were significantly lower, suggesting s
tasis and inefficient removal of perfusate in the side-by-side design.
The K,Values for water obtained in the continuous-flow chambers with
pig skin were similar to those published elsewhere for human skin.