C. Ernst et Hj. Rehm, UTILIZATION OF CHLORINATED S-TRIAZINES BY A NEW STRAIN OF KLEBSIELLA-PNEUMONIAE, Applied microbiology and biotechnology, 42(5), 1995, pp. 763-768
A bacterium utilizing 2-Chloro-4,6-diamino-s-triazine (CAAT) as sole n
itrogen source was isolated under a N-2-free atmosphere and identified
as Klebsiella pneumoniae. Concomitant to CAAT degradation the protein
content increased and chloride was released into the medium. Under ai
r and a N-2-atmosphere no reduction of CAAT degradation resulted, thou
gh this strain is able to fix molecular nitrogen, but the decompositio
n accelerated under anaerobic conditions. The degradation rate increas
ed continuously with increasing CAAT concentration. A continuous CAAT
degradation without CAAT accumulation was possible up to a influx rate
of 4.8 mu mol.1(-1)h(-1) (dilution rate = 0.007h(-1)). K. pneumoniae
A2 was also able to utilize deethylsimazine (CEAT) and deethylatrazine
(CIAT) as nitrogen source. Both under aerobic and anaerobic condition
s CEAT could be degraded faster than CIAT. The degradation sequence of
mixed s-triazines was cyanuric acid < CAAT < CEAT < CIAT, which was r
eflected by the degradation times of single compounds. Complete degrad
ation was assumed for all investigated s-triazine derivatives.