GENOTYPING OF THE GRANULOCYTE-SPECIFIC NA ANTIGENS FROM SMALL QUANTITIES OF BLOOD OR SERUM

To avoid the well-known shortcomings of phenotyping granulocytes for t he NA antigens using NA-specific human sera, a DNA-based method to det ermine the NA genotype was developed. Genomic DNA was isolated from bl ood cells or serum, amplified by polymerase chain reaction (PCR), immo bilized on nylon membrane and genotyped using digoxigenin-labeled, seq uence-specific oligonucleotides (SSO). The genotyping results of whole blood samples from 54 and of serum from 20 individuals correlated per fectly with our phenotyping using the antigen capture assay MAIGA. In three cases with the phenotype ''NA-null'' no hybridization of the NA- specific oligonucleotides occurred. These data show that SSO is a reli able method for NA genotyping especially if only small volumes of bloo d or even only serum probes are available.