SOME EXAMPLES OF THE USE OF THIN-LAYER SPECTROELECTROCHEMISTRY IN THESTUDY OF ELECTRON-TRANSFER BETWEEN METALS AND ENZYMES

Some examples of the use of optically transparent thin layer electrode s to study electron transfer between platinum or carbon and enzymes ar e given. With respect to the biomolecule, thin layer spectroelectroche mistry has been used to choose the best electrochemical pretreatment n ecessary to promote the heterogeneous electron transfer (in the case o f lactate dehydrogenase and glucose oxidase), to visualize intermediat es in electrochemical reactions (in the case of glucose oxidase and ho rseradish peroxidase), to determine the electron transfer rate constan t between oxidized, intermediate and reduced forms, and to prepare int ermediates compounds (in the case of compound III of horseradish perox idase), or to regenerate by electrochemical means enzymatic cofactors (in the case of hydrogenase).