APPLICATIONS OF SUPERCRITICAL CO2 EXTRACTION TO MICROALGAE AND PLANTS

A flow apparatus was constructed to carry out studies of supercritical fluid extraction in the temperature range 298.15-353.15 K and pressur es up to 40 MPa. To test the apparatus, studies on the solubility of n aphthalene in CO2 at 308.15, 313.15 and 318.15 K and pressures up to 3 5 MPa were carried out. These experimental data were correlated throug h the Peng-Robinson equation of state. Samples of the microalgae Botry ococcus braunii and Chlorella vulgaris which produce diolefines C-25-C -31 and carotenoids, respectively, were submitted to supercritical CO2 . The extraction yields for these compounds were studied and compared with those obtained using organic solvents. Supercritical CO2 was also used to produce extracts from rosemary leaves (Rosmarinus officinalis L.), which were compared with those obtained by hexane extraction.