REGENERATION OF FERTILE PLANTS FROM CELL-SUSPENSION-DERIVED SMALL-CELL COLONIES OF LOLIUM-PERENNE (L)

A defined method for the rapid establishment of cell suspensions relea sing small-cell colonies and their regeneration to fertile plants is d escribed. Cell suspensions were obtained from 10 mature, lengthwise-di vided embryos. Five of the 10 suspensions regenerated green plants. Fi ltration of suspensions through a 150 mu m mesh followed by regenerati on was carried out 28 days after initiation of the suspensions, to dem onstrate a rapid release of regenerable small cell colonies from cell suspensions using the protocol described. Two of the 10 filtered suspe nsions regenerated green plants from small cell colonies.