LONG-TERM STUDIES OF FUNGICIDE CONCENTRATIONS IN GREENHOUSES .1. TECHNIQUE FOR DETERMINING SURFICIAL FOLIAR RESIDUES OF FUNGICIDES WITH VINCLOZOLIN AND TRIADIMEFON AS MODEL COMPOUNDS
M. Papantoni et al., LONG-TERM STUDIES OF FUNGICIDE CONCENTRATIONS IN GREENHOUSES .1. TECHNIQUE FOR DETERMINING SURFICIAL FOLIAR RESIDUES OF FUNGICIDES WITH VINCLOZOLIN AND TRIADIMEFON AS MODEL COMPOUNDS, Journal of agricultural and food chemistry, 43(1), 1995, pp. 157-164
A new analysis procedure for fungicides has been developed gearing tow
ard estimation of worker exposure in greenhouses. The procedure includ
es sampling on leaves, glass, and polymers using vinclozolin and triad
imefon as model substances and starts with solvation of the fungicides
with ethanol from the surface. It proceeds by collection of the solut
ion on disks of cloth, disk extraction with excess of ethanol, evapora
tion of the solution to dryness, and addition of 1-chlorobutane and en
ds finally with HPLC determination. Validations of the evaporation ste
p, the desorption of analyte from the disks, and the whole procedure w
ere performed separately. The whole procedure gave recoveries of vincl
ozolin and triadimefon on leaves of 74% (RSD 4.2%) and 79% (RSD 5.0%),
respectively, and on hard surfaces around 90% (RSD ca. 6 %) for both
substances. The losses in the evaporation and in the desorption step w
ere small, the major losses occurring when the fungicide was collected
from the surface. Also, some degradation products of vinclozolin and
triadimefon, i.e., 3,5-dichloroaniline, triadimenol, and p-chloropheno
l, were briefly studied.