LOCALIZATION OF THE GENE FOR A NOVEL HUMAN ADENYLYL-CYCLASE (ADCY7) TO CHROMOSOME-16

A novel form of human adenylyl cyclase (ADCY7) has been discovered in the human erythroleukemia cell line (HEL). This cell line has been wid ely used as a model for studies of the characteristics of human platel ets. Data from HEL cells suggests that ADCY7 may the major AC form in human platelets. In the current study polymerase chain reaction (PCR) techniques coupled with use of human/rodent somatic hybrid panels and a yeast artificial chromosome (YAC) library were used to determine the chromosomal localization of the gene (adcy7) for ADCY7 enzyme. A 251- bp product from the 3' untranslated region of human adcy7 was amplifie d for PCR mapping and the results localize the adcy7 gene to region 16 q12-16q13 of the human genome. The AC enzyme family is characterized b y the presence of 12 membrane-spanning domains in its sequences, and t his chromosomal region is known to contain other genes codding for pro teins characterized by 13 membrane-spanning domains.