I. Crandall et al., PLASMODIUM-FALCIPARUM - CD36 DEPENDENT CYTOADHERENCE OR ROSETTING OF INFECTED ERYTHROCYTES IS MODULATED BY KNOBS, Cell adhesion and communication, 2(6), 1994, pp. 503-510
A knobless (K-) line of the FCR-3 isolate of Plasmodium falciparum was
obtained by gelatin flotation. Immunofluorescent staining and immunob
lots indicated that both the K- line and the K+ (knobby) line from whi
ch it was derived contained similar forms of potentially adhesive modi
fied band 3 protein. When the K+ and K- lines were assayed for their c
ytoadherent and resetting abilities the K+ line showed a high level of
CD36 dependent cytoadherence, whereas the K- line demonstrated a mark
ed pH dependent increase in resetting. Resetting was inhibited by the
addition of peptides based on band 3 motifs, suggesting that cytoadher
ence and resetting involve the same adhesin but that the presence of k
nobs affects whether the adherent preference of the infected erythrocy
te is uninfected red cells or endothelial/C32 amelanotic melanoma cell
s.