We improved the techniques for determining prey items based on hair ch
aracteristics from seats of the free-ranging Asiatic lion Panthera leo
persica. (Meyer, 1826) in terms of accuracy and time efficiency. Cuti
cular characteristics of hair are similar in many Indian prey species
and are not species specific. A combination of hair characteristics, e
.g. hair width, medullary structure, and medulla width expressed as pe
r cent of hair width, are most useful to identify the common mammalian
prey species. We found that examination of a minimum of 20 hairs/scat
and 30 seats would provide a reliable estimate of the lion's diet bas
ed on the per cent occurrence of prey species hair in the seats.