NICOTINAMIDE ADENINE-DINUCLEOTIDE REGULATES MUSCARINIC RECEPTOR-COUPLED K-15 CELLS( (M) CHANNELS IN RODENT NG108)

1. The possible role of nicotinamide-adenine dinucleotide (NAD(+)) and cyclic adenosine diphosphate ribose (cADPR) as regulators of M-type K + currents (I-K(M) has been studied in whole-cell patch-clamped NG108- 15 mouse neuroblastoma x rat glioma cells that had been transformed to express mi muscarinic acetylcholine receptors (mAChRs). 2. Pre-incuba tion of NG108-15 cells for 6-8 h with streptozotocin (2-5 mM) reduced NAD(+) levels by 40-50%. Nicotinamide (2-5 mM increased NBD+ levels an d prevented depletion by streptozotocin. 3. Streptozotocin pretreatmen t reduced the inhibition of produced by 100 mu M acetylcholine (ACh) f rom 51.6 +/- 7.0 to 29.1 +/- 7.5%. This was prevented by simultaneous pre-incubation with 2 mM nicotinamide or by adding 2 mM NAD(+) to the pipette solution. Neither procedure significantly affected the initial amplitude of IK(M). 4. Inclusion of 2 mu M cADPR in the pipette solut ion induced a slow loss of I-K(M) with a time constant of about 20 min . 5. It is concluded that mAChR-induced inhibition of I-K(M) requires intracellular NAD(+). This might be needed for the formation of cADPR as a regulator or messenger for I-K(M) inhibition.