C. Ravel et al., CONSERVATION AMONG OLD-WORLD LEISHMANIA SPECIES OF 6 PHYSICAL LINKAGEGROUPS DEFINED IN LEISHMANIA-INFANTUM SMALL CHROMOSOMES, Molecular and biochemical parasitology, 69(1), 1995, pp. 1-8
We have characterised 49 DNA probes specific for each of the six small
est chromosomes in Leishmania infantum and have examined the allocatio
n of these probes in the molecular karyotypes of the other Old World L
eishmania species Leishmania donovani, Leishmania major, Leishmania tr
opica and Leishmania aethiopica. These 49 probes define 6 physical lin
kage groups in the molecular karyotypes of various strains of L. infan
tum. 40 of these probes hybridise in the other Old World Leishmania sp
ecies and show a remarkably conserved linkage pattern. No interchromos
omal exchange nor fusion could be detected. Thus, in spite of the chro
mosomal size polymorphisms, the general structure of the genome seems
to be conserved in the six smallest chromosomes among Old World Leishm
ania species. This structural genomic homogeneity should be helpful fo
r mapping studies of any Old World Leishmania genomes.