Somatic embryogenesis was obtained when stem explants of Aesculus hipp
ocastanum L. were cultured in vitro on agar-solidified MS medium suppl
emented with 9.3 mu M kinetin, 10.7 mu M 1-naphthaleneacetic acid and
9.0 mu m 2,4-dichlorophenoxyacetic acid. Plantlets were obtained in gr
owth regulator-free medium.