EFFECTS OF FLUOXETINE ON BASAL AND K-INDUCED TRITIUM RELEASE FROM SYNAPTOSOMES PRELOADED WITH [H-3] SEROTONIN()

Synaptosomes from rat brain cortex and spinal cord were preloaded with [H-3]serotonin ([H-3]5-HT), superfused and exposed to fluoxetine and/ or 15 mM K+. In both regions 10 mu M, but not 1 mu M fluoxetine evoked a marked tritium overflow, about 2 min later than the immediate [H-3] 5-HT release induced by K+, and mainly (73%) due to the efflux of a tr itiated metabolite of 5-HT, possibly [H-3]5-hydroxy-indoleacetic acid. These findings confirm previous data in the rat hippocampus and are p robably due to fluoxetine interacting with the 5-HT storage vesicles. One mu M fluoxetine significantly reduced the d-fenfluramine-induced [ H-3]5HT overflow, in accordance with its action as 5-HT uptake blocker , but did not affect the K+-induced [H-3]5-HT overflow. This latter fi nding does not confirm that fluoxetine inhibits the depolarization-ind uced Ca2+-influx, suggested to involve a drug interaction with the L-t ype Ca2+-channels. This, the overflow induced by 10 mu M fluoxetine wa s additive with the depolarization-induced overflow, when the two stim uli were applied together. When 10 mu M fluoxetine was added 7 min bef ore 15 mM K+, there was no depolarization-induced overflow. Such inhib ition might be only apparent and due either to the fluoxetine-induced loss of vesicular 5-HT or to a fluoxetine-induced alteration of synapt ic vesicles. The in vivo relevance of the fluoxetine releasing effect remains to be assessed.