A RAPID DETECTION AND IDENTIFICATION OF HEMORPHINS RELEASED FROM BOVINE HEMOGLOBIN ENZYMATIC-HYDROLYSIS BY USE OF HPLC COUPLED WITH PHOTODIODE-ARRAY DETECTOR

Identification of hemorphins issued from a complex hemoglobin enzymati c hydrolysate was carried out by UV-spectra comparison. Two hemorphins , VV-hemorphin-7 and LVV-hemorphin-7, were detected in a single step b y the use of HPLC coupled with photodiode array detector. This techniq ue greatly simplified the the multistage identification and purificati on strategy. This method could also be efficiently applied to the iden tification of peptides containing aromatic amino acids.