DEVELOPMENT OF A HPLC METHOD FOR THE DETERMINATION OF 17-BETA-ESTRADIOL-3-PHOSPHATE IN PHARMACEUTICAL PREPARATIONS

Authors
MILLER RB DELKER G CHEN CA SHERWOOD CH
Citation
Rb. Miller et al., DEVELOPMENT OF A HPLC METHOD FOR THE DETERMINATION OF 17-BETA-ESTRADIOL-3-PHOSPHATE IN PHARMACEUTICAL PREPARATIONS, Journal of liquid chromatography, 18(1), 1995, pp. 127-136
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
18
Issue
1
Year of publication
1995
Pages
127 - 136
Database
ISI
SICI code
0148-3919(1995)18:1<127:DOAHMF>2.0.ZU;2-9
Abstract
A validated reversed-phase high-performance liquid chromatographic (HP LC) procedure for the analysis of 17 beta-estradiol-3-phosphate is rep orted. In the development of this assay, several factors were evaluate d including buffer ionic strength, mobile phase pH, ion-pairing concen tration, organic composition, and column type. The described method is rapid and coupled with standard HPLC procedures leads to a selective, accurate, and reproducible assay. The peak area versus 17 beta-estrad iol-3-phosphate concentration is linear over the range of 0.1 - 100 mu g/mL, with a detection limit of 0.02 mu g/mL.