IDENTIFICATION OF 2 CELL-CYCLE-REGULATED GENES AFFECTING THE BETA-1,3-GLUCAN CONTENT OF CELL-WALLS IN SACCHAROMYCES-CEREVISIAE

The Calcofluor white-hypersensitive mutants cwh52 and cwh53 are severe ly reduced in beta 1,3-glucan. CWH52 was equivalent to GAS1. CWH53 rep resented a new gene, located on the right arm of chromosone XII, and p redicted to encode a 215 kDa protein with multiple transmembrane domai ns. The transcription of CWH53 was cell cycle-dependent and, similar t o GAS1/CWH52, increased in late G(1), indicating that the formation of beta-glucan is cell cycle-regulated. Further, in some mutant alleles of both gas1/cwh52 and cwh53 lethal concentrations of Calcofluor induc ed growth arrest at a specific phase of the cell cycle.