ACTIVATION SIGNALS ARE DELIVERED THROUGH 2 DISTINCT EPITOPES OF CD100, A UNIQUE 150-KDA HUMAN LYMPHOCYTE SURFACE-STRUCTURE PREVIOUSLY DEFINED BY BB18-MAB
C. Herold et al., ACTIVATION SIGNALS ARE DELIVERED THROUGH 2 DISTINCT EPITOPES OF CD100, A UNIQUE 150-KDA HUMAN LYMPHOCYTE SURFACE-STRUCTURE PREVIOUSLY DEFINED BY BB18-MAB, International immunology, 7(1), 1995, pp. 1-8
We have previously reported the identification of a glycoprotein dimer
, now termed CD100, at the lymphocyte cell surface using a mAb termed
BB18. In the present study we isolated a second mAb, termed BD16, reco
gnizing another epitope of this 150 kDa structure. We show that intera
ction with individual mAb triggers differential functional effects on
peripheral blood lymphocytes (PBL) and purified T cells. In particular
and of the utmost interest, BD16 mAb strongly inhibits CD3 induced PB
L proliferation while it dramatically increases the CD2 induced prolif
eration. In contrast, after T cell purification, BD16 mAb increases bo
th CD2 and CD3 induced proliferation. It has to be noted that perturba
tion of the same 150 kDa surface molecule with BB18 mAb exerts no effe
ct on CD2 and CD3 induced proliferations, while inducing a strong lymp
hocyte proliferation in the presence of submitogenic concentrations of
phorbol myristate acetate.