HPLC ANALYSIS OF METRONIDAZOLE AND DILOXANIDE FUROATE IN ITS DOSAGE FORMS

A high performance liquid chromatographic method for the simultaneous determination of metronidazole and diloxanide furoate in single and co mbined dosage form has been developed. Each drug acted as internal sta ndard for the other. Both of the drugs eluted from the cyclobond colum n (beta-cyclodextrin stationary phase) without retardation effects, us ing methanol-0.05 M NaH2PO4 (pH 7) in ratio 35:65, flow rate 1 ml min( -1) and detection at 254 nm. Metronidazole and diloxanide furoate gave rectilinear calibration graphs in the ranges 1-10 mu g and 1-5 mu g r espectively. Recoveries were in the range 99.6 to 101.3% and their cor responding coefficient of variation are 0.7 and 1.05% (n=6).