SARCOCYSTIS-FUSIFORMIS - SOME KREBS CYCLE ENZYMES IN VARIOUS FRACTIONS OF SARCOCYSTS OF BUFFALO (BUBALUS-BUBALIS)

A biochemical investigation was carried out on the relative presence o f some enzymes of the Krebs cycle and of the associated energy metabol ism in various fractions (namely, cyst wall, cyst fluid and zoites) of sarcocysts of Sarcocystis fusiformis from the oesophageal muscles of naturally infected Indian water buffalo (Bubalus bubalis). Except for malate dehydrogenase, the activities of aconitase, isocitrate dehydrog enase, succinate dehydrogenase and fumarase were beyond detectable lim its, pointing to a non-functional Krebs cycle in the cysts of this par asite. The activities of adenosine triphosphatase and cytochromes were lowest is cyst fluid and were maximally depicted by cyst wall and zoi tes.