D. Dudorkinova et al., APPLICATION OF NADH TETRAZOLIUM REDUCTASE REACTION IN PERIOPERATIVE BIOPSY OF DYSGANGLIONIC LARGE-BOWEL, European journal of pediatric surgery, 4(6), 1994, pp. 362-365
A reaction for NADH tetrazolium reductase was used to demonstrate the
presence of ganglion cells in myenteric plexus during dysganglionic bo
wel operations in 24 patients. The advantages of this method are the q
uick preparation of an incubation medium, a short incubation time and
the good demonstration of ganglion cells. It was possible to determine
the extent of the aganglionic and prominently hypoganglionic parts of
the bowel and in this way achieve improved results. The NADH tetrazol
ium reductase reaction can be recommended for the perioperative biopsy
of a dysganglionic bowel.