AN IMPROVED METHOD TO DISTINGUISH MICROCOCCAL NUCLEASE SENSITIVITY OFCHROMATIN

Authors
HAMID QA THANUMALAYAN S PARNAIK VK
Citation
Qa. Hamid et al., AN IMPROVED METHOD TO DISTINGUISH MICROCOCCAL NUCLEASE SENSITIVITY OFCHROMATIN, Journal of biochemical and biophysical methods, 33(1), 1996, pp. 59-64
Citations number
16
Categorie Soggetti
Biology,Biophysics,"Biochemical Research Methods
Journal title
Journal of biochemical and biophysical methods → ACNP
ISSN journal
0165022X
Volume
33
Issue
1
Year of publication
1996
Pages
59 - 64
Database
ISI
SICI code
0165-022X(1996)33:1<59:AIMTDM>2.0.ZU;2-3
Abstract
The resolution of the standard micrococcal nuclease assay for sensitiv ity of active chromatin has been enhanced by the inclusion of an addit ional step of digesting nuclease-digested DNA with a suitable restrict ion enzyme prior to Southern hybridization. The improved assay has bee n used to analyze the chromatin structure of the lamin A, albumin and alpha-fetoprotein genes during rat liver development.