PHOSPHOLIPASE-C GAMMA-2 (PLCG2) AND PHOSPHOLIPASE-C GAMMA-1 (PLCG1) MAP TO DISTINCT REGIONS IN THE HUMAN AND MOUSE GENOMES

The phospholipase C gamma-2 (Plcg2) gene encodes an enzyme that plays a crucial role in intracellular signal transduction pathways. This enz yme is important be cause of its role in the generation of second mess engers following the hydrolysis of phosphatidylinositol 4,5-bisphospha te. We have now determined the chromosomal location of this gene in th e mouse and human genomes. An interspecific backcross involving AEJ/Gn and Mus spretus mice was used to localize the gene in mouse. A rodent /human somatic cell hybrid panel was used to map PLCG2 in the human ge nome. Our results position PLcg2 in the central region of mouse chromo some 8. We also show that PLCG2 maps to the long arm of human chromoso me 16, in the region q22-qter. PLcg2 does not map near its most closel y related family member, PLcg1, in either genome, indicating that the mammalian Plcg genes belong to a dispersed family. (C) 1995 Academic P ress, Inc.