A 500-KILOBASE REGION CONTAINING THE TUBEROUS SCLEROSIS LOCUS (TSC1) IN A 1.7-MEGABASE YAC AND COSMID CONTIG

A complete overlapping clone map of a 1.7-Mb region from DBH to D9S67 that includes the TSC1 candidate region has been constructed. The map includes YAC and cosmid clones, contains STS approximately every 50 kb on average, and establishes the order of five previously unordered lo ci, The overall physical length of this segment of chromosome 9q34 (1. 7 Mb) is significantly less than expected compared to its estimated ge netic length (similar to 10 cM). Consequently, the physical length of the TSC1 candidate region is substantially less than predicted by a ge netic distance of similar to 2 cM. (C) 1995 Academic Press, Inc.