HIGH-RESOLUTION CYTOGENETIC MAPPING OF THE SHORT ARM OF CHROMOSOME-1 WITH NEWLY ISOLATED 411-COSMID MARKERS BY FLUORESCENCE IN-SITU HYBRIDIZATION - THE PRECISE ORDER OF 18 MARKERS ON 1P36.1 ON PROPHASE CHROMOSOMES AND STRETCHED DNAS

A high-resolution cytogenetic map of the short arm of chromosome 1 wit h newly isolated 411 cosmid markers was constructed by fluorescence in situ hybridization (FISH). These markers were scattered throughout ch romosome 1p, but they were preferentially concentrated on R-band domin ant regions such as 1p36, 1p34, 1p32, 1p22, and 1p13. Among these mark ers, 197 were localized on chromosome band 1p36, a region frequently d eleted in neuroblastoma. Of these, 18 were precisely ordered on 1p36.1 by multicolor FISH of prophase chromosomes and ''stretched'' DNAs as follows: 8-614-631-665-451-199-190-561-241-74-176-652-1cen. The high-d ensity map of chromosome 1p constructed here can provide useful landma rks for constructing a contig map of the short arm of chromosome 1 wit h YACs and cosmid clones and will expedite the identification of break points and/or tumor suppressor gene(s) associated with several types o f malignant tumors that frequently exhibit chromosomal aberrations or deletions of chromosome 1p. (C) 1995 Academic Press, Inc.