THE MOUSE FAU GENE - GENOMIC STRUCTURE, CHROMOSOMAL LOCALIZATION, ANDCHARACTERIZATION OF 2 RETROPSEUDOGENES

The Fau gene is the cellular homolog of the fox sequence of the Finkel -Biskis-Reilly murine sarcoma virus (FBR-MuSV). FBR-MuSV acquired the Fau gene by transduction in a transcriptional orientation opposite to that of the genomic Fau gene. The genomic structure of the mouse Fau g ene (MMFAU) and its upstream elements have been determined and are sim ilar to those of the human FAU gene, The gene consists of five exons a nd is located on chromosome 19. The first exon is not translated, The promoter region has no well-defined TATA box but contains the polypyri midine initiator flanked by regions of high GC content (65%) and shows all of the characteristics of a housekeeping gene. The 5' end of the mRNA transcript was determined by 5' RACE analysis and is located, as expected, in the polypyrimidine initiator site. Furthermore, the seque nces of two retropseudogenes (Fau-ps1 and Fau-ps2) are reported. Both pseudogenes are approximately 75% identical to the Fau cDNA, but both are shorter due to a deletion at the 5' end and do not encode a functi onal protein, Fau-prs is interrupted by an AG-rich region of about 350 bp within the S30 region of the Fau cDNA. Fau-ps1 was localized on ch romosome 1 and Fau-ps2 on chromosome 7. (C) 1995 Academic Press, Inc.