TRANSESTERIFICATION BETWEEN TRIOLEIN AND ETHYLBEHENATE BY IMMOBILIZEDLIPASE IN SUPERCRITICAL CARBON-DIOXIDE

Transesterification between triolein (OOO) and ethylbehenate (EB) was carried out to produce 1,3-dibehenoyl-2-oleoyl glycerol. (BOB), an ant iblooming agent for chocolate, by 1,3-regiospecific immobilized lipase (Lipozyme IM) in supercritical carbon dioxide (SCCO2). An increase in the concentration of EB accelerated the transesterification activity to BOB while an increase in the concentration of 000 was inhibitory, s uggesting that the transesterification proceeds via behenoyl-enzyme co mplex. The optimum water content in immobilized enzyme for transesteri fication increased with an increase in the initial substrate concentra tion. The optimum temperature and pressure for the maximum productivit y of BOB were determined to be 50 degrees C and 15 MPa, respectively, as determined from both the initial period activity and the reaction e quilibrium.