Recipient exposure to allogeneic donor leukocytes can mediate a number
of immunologic complications of transfusion or can transmit leukotrop
ic viruses carried by the donor. Leukocyte depletion of cellular blood
components has been shown to reduce the incidence of such complicatio
ns. In recent years, prestorage leukocyte depletion by filtration has
also been suggested as a means of decreasing the incidence of bacteria
l overgrowth in cellular blood components. This review analyzes publis
hed studies on the use of leukodepletion filters for removal of Staphy
lococcus epidermidis and Yersinia enterocolitica from blood. Although
ineffective for removal of S. epidermidis from Platelet Concentrates,
inoculation studies demonstrate removal of low levels of Y. enterocoli
tica from Red Cell Concentrates. Based on these studies, four possible
mechanism(s) for removal of bacteria by leukodepletion filters are an
alyzed: phagocytosis by leukocytes during a prefiltration holding peri
od; complement-mediated bacterial killing enhanced by filtration; adhe
rence of bacteria to leukocyte surfaces retained within the filter; an
d direct removal of bacteria by the filter media. Just as multiple mec
hanisms appear to account for the efficiency with which these filters
deplete blood of leukocytes, it is likely that more than one mechanism
accounts for the experimental observation that leukocyte depletion fi
lters can reduce overgrowth of Y. enterocolitica in stored Red Cell Co
ncentrates.