IMMUNOCHEMICAL DETECTION OF METHAMPHETAMINE USING POLYMER-PROTECTED ULTRAFINE PLATINUM PARTICLES

Ultrafine platinum particles protected by poly (methyl acrylate-co-N-v inyl-2-pyrrolidone) were applied to the immunological detection of met hamphetamine (MA). The polymer-protected ultrafine particles could che mically bind anti-methamphetamine monoclonal antibody to their surface s. The antibody-fixed particles behaved like an antibody in the immuno reaction. The antibody-bound particles competitively reacted against t he immobilized and the free antigens in the ELISA system. MA could be detected down to a concentration of ca. 10 ng/ml. In the immune-dot as say, 10-100 ng/ml of MA was detected within 30 min using the ultrafine particles.