EPITOPE TAG VECTORS FOR EUKARYOTIC PROTEIN-PRODUCTION

We report on the construction and use of two eukaryotic expression vec tors which add well-characterized epitope tags to the N termini of pro teins. The utility of these Vectors is demonstrated for detecting the expression of a variety of proteins. As the addition of these epitope tags can in some cases obviate the need to generate specific antisera to each individual protein, these vectors provide a facile means both to monitor protein expression and to purify such expressed proteins.