V. Desaiyajnik et al., THE EFFECT OF THYROID-HORMONE TREATMENT ON THE GENE-EXPRESSION AND ENZYME-ACTIVITY OF RAT-LIVER SODIUM-POTASSIUM DEPENDENT ADENOSINE-TRIPHOSPHATASE, Endocrinology, 136(2), 1995, pp. 629-639
The effects of thyroid hormone (T-3) treatment on liver Na,K-adenosine
triphosphatase (Na,K-ATPase) at the levels of subunit messenger RNA (
mRNA), enzymatic activity, and enzyme content were studied in euthyroi
d rats injected for 5 consecutive days with T-3. Northern and slot blo
t analyses of polyadenylated mRNA revealed that T-3 treatment coordina
tely increases the level of mRNA encoding the alpha(1)- and beta(1)-su
bunits, approximately 4- and 3-fold, respectively, above basal levels.
To determine whether this increase in the subunit mRNA consequently r
esults in an increase in the synthesis of the enzyme, a modified liver
cell fractionation procedure was developed, and the subcellular fract
ions from control and T-3-treated livers were examined biochemically.
Western blot analysis and Na,K-ATPase assay demonstrated that T-3 trea
tment resulted in a 2-fold increase in both the amount and activity of
the enzyme. Furthermore, the Western blot analysis of endoglycosidase
-H-treated membrane fractions revealed an increase in the amount of th
e precursor beta-subunit in the T-3-treated liver rough microsomal fra
ction, suggesting that an increase in subunit synthesis contributes at
least partially to the increase in the rat liver Na,K-ATPase by T-3 t
reatment.