SIMPLE, RAPID AND SENSITIVE SPECTROFLUOROMETRIC DETERMINATION OF DIFLUNISAL IN SERUM AND URINE BASED ON ITS TERNARY COMPLEX WITH TERBIUM AND EDTA

Authors
IOANNOU PC LIANIDOU ES KONSTANTIANOS DG
Citation
Pc. Ioannou et al., SIMPLE, RAPID AND SENSITIVE SPECTROFLUOROMETRIC DETERMINATION OF DIFLUNISAL IN SERUM AND URINE BASED ON ITS TERNARY COMPLEX WITH TERBIUM AND EDTA, Analytica chimica acta, 300(1-3), 1995, pp. 237-241
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
Analytica chimica acta → ACNP
ISSN journal
00032670
Volume
300
Issue
1-3
Year of publication
1995
Pages
237 - 241
Database
ISI
SICI code
0003-2670(1995)300:1-3<237:SRASSD>2.0.ZU;2-Y
Abstract
A very simple, rapid and highly sensitive fluorimetric method for the determination of diflunisal in serum and urine is described. The metho d is based on the formation of a ternary complex between diflunisal, T b3+ and EDTA in alkaline aqueous solutions. This complex exhibits very intense terbium ion luminescence with a main emission maximum at 546 nm when excited at 284 nm. Optimum conditions for the complex formatio n have been investigated. The detection limit for diflunisal is 2.4 mu g l(-1), while the range of application is 0.01-6.00 mg l(-1). The me thod has been successfully applied for the determination of diflunisal in untreated human serum and urine samples. Analytical recoveries fro m serum and urine samples spiked with diflunisal were in the ranges of 96.8-101.2% and 98.0-102.0%, respectively.