ENZYMATIC SPECTROPHOTOMETRIC DETERMINATION OF PHYTIC ACID WITH PHYTASE FROM ASPERGILLUS-FICUUM

Authors
MARCH JG VILLACAMPA AI GRASES F
Citation
Jg. March et al., ENZYMATIC SPECTROPHOTOMETRIC DETERMINATION OF PHYTIC ACID WITH PHYTASE FROM ASPERGILLUS-FICUUM, Analytica chimica acta, 300(1-3), 1995, pp. 269-272
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
Analytica chimica acta → ACNP
ISSN journal
00032670
Volume
300
Issue
1-3
Year of publication
1995
Pages
269 - 272
Database
ISI
SICI code
0003-2670(1995)300:1-3<269:ESDOPA>2.0.ZU;2-I
Abstract
A method to determine phytic acid in the range 3-60 mu M based on the spectrophotometric determination of inorganic phosphate with vanadate and molybdate, after Liberation by enzymatic hydrolysis of phytic acid with phytase from Aspergillus ficuum at pH 2.5 and 37 degrees C is re ported. The method has been applied successfully to determine phytic a cid in wheat flour and in a pharmaceutical product.