ENZYMATIC METHOD FOR THE AMPEROMETRIC DETERMINATION OF NICOTINIC-ACIDIN MEAT-PRODUCTS

An enzymic method for the determination of nicotinic acid is described , based on the indirect electrochemical monitoring of nicotinic acid v ia its reaction with oxygen in the presence of nicotinic acid hydroxyl ase. Derivative amperometric signals due to oxygen depletion enable a one-point kinetic analysis to be carried out, Nicotinic acid hydroxyla se catalyses the hydroxylation of nicotinic acid to produce 6-hydroxyn icotinic acid, which is accompanied by a stoichiometric consumption of oxygen. The method was applied successfully to the analysis of real s amples.