USE OF FLUORESCAMINE FOR THE SPECTROFLUOROMETRIC INVESTIGATION OF PRIMARY AMINES ON SILANIZED GLASS AND INDIUM TIN OXIDE-COATED GLASS

Fluorescamine reacts with primary amines to yield a fluorescent produc t, Treatment of aminosilanized glass and aminosilanized indium tin oxi de-coated glass allows the silanized surface to be investigated in sit u. Two methods of silanizing these materials were compared. The effect of aqueous solutions on the silanized surface was monitored, The resu lts were used to account for the amount of horseradish peroxidase cova lently attached to the surfaces, The N-hydroxysuccinimide ester of fer roceneacetic acid was prepared and covalently attached to aminosilaniz ed indium tin oxide-coated glass, and its electrochemical properties w ere studied with the aid of fluorescamine.