Rn. Crowhurst et al., RAPD CHARACTERIZATION OF FUSARIUM-OXYSPORUM ASSOCIATED WITH WILT OF ANGSANA (PTEROCARPUS-INDICUS) IN SINGAPORE, Mycological research, 99, 1995, pp. 14-18
Angsana (Pterocarpus indicus) is an important shade tree in Singapore.
Since 1914 angsana has been under threat from a wilt disease caused b
y Fusarium oxysporum. We have used the PCR based technique of Random A
mplification of Polymorphic DNA (RAPD) to assess the genetic variabili
ty of 39 isolates of F. oxysporum isolated from angsana. The isolates
formed a homogeneous group with few polymorphic bands. The most signif
icant polymorphic band distinguished four isolates obtained from one r
egion (the Istana) of Singapore from the other 35 isolates. This diffe
rence was supported by Southern analysis data using RAPD fragment OPA-
18(350) as a hybridization probe. Angsana isolates of F. oxysporum wer
e easily distinguishable from 17 other F. oxysporum isolates from othe
r hosts, and from ten isolates of F. solani from angsana.