SIMULTANEOUS DETERMINATION OF BUZEPIDE, PHENYLPROPANOLAMINE, AND CLOCINIZINE IN PHARMACEUTICAL PREPARATIONS BY ION-PAIR REVERSED-PHASE HPLC

A rapid, accurate and precise method for the determination of buzepide , phenylpropanolamine, and clocinizine in a cough-mixture is described . The method is based on reversed-phase ion-pair HPLC. The only sample preparation necessary for the analysis is its dilution with the mobil e phase. The resulting solution is analyzed on a column packed with Li chrosorb RP-18 (10 mu m) with acetonitrile in aqueous 5 10-2M sodium p erchlorate, pH 3.0 (15:85) as initial mobile phase (2 mL.min-1) and de tection at 220 and 254 nm. Then a linear gradient up to 95% acetonitri le in 20 min, is applied. The detection limits are 500 ng injected for phenylpropanolamine, 40 and 5 ng for buzepide and clocinizine, respec tively.