FURTHER INVESTIGATIONS IN HPLC SYSTEMS CONTAINING SILICA AND POLAR BONDED SILICA PACKING DYNAMICALLY MODIFIED BY CAMPHORSULFONIC ACID

The investigation of chromatographic (HPLC) systems containing camphor sulfonic acid (CSA) has been continued. Similarly to the previous find ing for silica, on polar bonded silica packings CSA forms double layer . The adsorbed amount of CSA was determined by the evaluation of break -through curves. It was found that the addition of triethylamine in a small excess to the CSA containing eluent results in the formation of a versatile chromatographic system. The adsorbed amount of CSA and TEA from such eluents was determined by the estimation of the break-throu gh curves were gained by simultaneous refractometric and UV spectropho tometric detection. The migration of the compounds follows a normal ph ase mechanism evidenced by the behavior of the examined medicinal comp ounds.