RETENTION DEPENDENCE ON ORGANIC MODIFIER AND INTERACTION REAGENT CONCENTRATION IN REVERSED-PHASE ION-INTERACTION HPLC

The effect played on ion-interaction chromatographic retention by the concentrations of the organic modifier and of the ion-interaction reag ent as a function of the ionic strength is studied. Experiments are pe rformed for a series of analytes characterized by different chemical p roperties (nitrate, nitrite, iodide, ascorbic, erotic and p-aminobenzo ic acids, aniline, benzylamine, p- and m-aminophenol) in the absence a nd in the presence of organic modifiers (acetonitrile and methanol) an d of sodium perchlorate to control the ionic strength(I = 1.0 M).