ANALYSIS OF TRIACYLGLYCEROLS OF BORAGE OIL BY RPLC IDENTIFICATION BY COINJECTION

Borage oil is an interesting oil because it is rich in gamma linolenic acid (gamma Ln 18:3 Z6, Z9, Z12). However, since triacylglycerol stan dards with gamma Ln chains do not exist, there is a problem identifyin g these particular triacylglycerols. Because the hyphenated techniques (GC-MS, LC-MS) do not give sufficient resolution for unequivocal iden tification, an easier methodology is described here. It consists of us ing another oil of known composition as a standard mixture. Use of thi s method is shown for the assignment of triacylglycerols in borage oil .